A, sum of the integrals for 60 Hz, 200 impulses at 9 mA to 32 mA constant current field stimulation. Small amounts of ATP were detected at 9 mA to 30 mA, but the best amperage was 32 mA for detection of ATP. B, the sum of the integrals for different frequencies (200 impulses, 32 mA). The best frequency for detection of ATP was 60 Hz.