Figure 6. Summary of the results from adding tetrodotoxin to determine the neural component of ATP overflow (n = 2, 6-month-old rats).

ATP overflow increased briefly following field stimulation with no blocking (A). After the addition of the vehicle (citrate buffer) for tetrodotoxin, ATP overflow increased significantly between 10 and 45 s following field stimulation (B). After the tetrodotoxin was added, no ATP overflow was detected following field stimulation (C).