Table 3.
Holding solution and additives
| Additive | Type of study | Incubation time | Result |
|---|---|---|---|
| ATP-MgCl (energy source) | In vitro | 5 h in UNS at 26°C | ↑Survival in culture at 10 days |
| Aminoguanidine (inhibitor of iNOS) | In vitro | 5 h in DMEM with HEPES at 26°C | ↑HSE ↓cHADF |
| 14,15 epoxyeicosatrienoic acid (inhibits arachidonic acid) | In vitro | 5 h in DMEM with HEPES at 26°C | ↑HSE ↓cHADF |
| Vitamin B12 (inducer of cell proliferation) | In vitro | 5 h in PBS at 26°C | ↑HSE Induction of β-catenin transcription |
| Aminoguanidine (inhibitor of iNOS) | In vitro | 3 h in TCM at 26°C | No post-operative shedding (0/6) |
| PRP (contains growth factors) | In vitro | 15 min | 15% ↑ in graft survival |