Table 1.
Kinetic parameters of CYP102A2 using SDS and NADPH as substrates. Steady-state kinetic measurements were performed at 37°C in 0.1 M potassium phosphate buffer, pH 7.2. All initial velocities were determined in triplicate. The kinetic parameters K cat and K m for NADPH were calculated by nonlinear regression analysis of experimental steady-state data using the GraFit (Erithacus Software Ltd.) program. The V max value, S 0.5, and the Hill coefficient, n H, for SDS were determined by fitting the plotted v versus substrate concentration to the Hill equation using the GraFit (Erithacus Software Ltd.) program.
| NADPH | ||
|---|---|---|
| k cat (s−1) | K m(μM) | K cat/K m (μM−1∗s−1) |
| 2.05 ± 0.05 | 8.30 ± 0.62 | 0.25 |
|
| ||
| SDS | ||
|
| ||
| S 0.5 (mM) | V max (μmol/min) | n H |
| 1.22 ± 0.04 | 0.18 ± 0.0111 | 5.7 ± 0.66 |
|
| ||
| Lauric acid | ||
|
| ||
| S 0.5 (mM) | V max (μmol/min) | n H |
| 0.47 ± 0.005 | 0.088 ± 0.003 | 8.7 ± 0.57 |