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Effect of inhibitors on activity of keratinase.
| S. no. | Concentration (mM) | Residual activity (%) | |||
|---|---|---|---|---|---|
| PMSF | EDTA | DTT | β-mercaptoethanol | ||
| (1) | 1 | 5.00 | 74.00 | 120.00 | 240.00 |
| (2) | 5 | 0.00 | 70.00 | 158.00 | 609.00 |
| (3) | 10 | 0.00 | 70.00 | 203.00 | 876.00 |
Control without inhibitors was taken as 100% activity corresponded to 720 U/mL on feather keratin as substrate.
