Table 5.
Kinetic parameters of purified keratinase on casein and pNA.
| Substrates | Km | V max | Kcat (min−1) |
|---|---|---|---|
| Casein | 0.25 mg/mL | 0.75 μg/mL/min | 3.0 |
| N-Suc-Ala-Ala-Pro-Phe-pNA | 0.1 mM | 2.0 μmoles/mL/min | 20.0 |
| N-Ala-Ala-Pro-Leu-pNA | 0.25 mM | 3.0 μmoles/mL/min | 12.0 |
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