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(a) Native PAGE of the purified L-asparaginase from the E. carotovora, (i) molecular weight marker proteins, (ii) purified asparaginase 10 μgm/mL, (iii) purified asparaginase 25 μgm/mL. (b) SDS-PAGE of the purified L-asparaginase from the E. carotovora, lane 1 molecular weight marker proteins, lane 2 purified asparaginase after DEAE sephadex chromatography, lane 3 purified asparaginase after CM Cellulose chromatography, lane 4 crude preparation.
