Table 2.
Influence of different metal ions and reagents on L-asparaginase activity.
| Addition | Concentration (mM) | Relative activity (%) |
|---|---|---|
| No addition | none | 100 |
| Na+ (NaCl) | 1.0 | 117 |
| K+ (KCl) | 1.0 | 133 |
| Mg2+ (MgCl2) | 1.0 | 24 |
| Ca2+ (CaCl2) | 1.0 | 78 |
| Mn2+ (MnCl2) | 1.0 | 71 |
| Zn2+ (ZnCl2) | 0.5 | 06 |
| Fe3+ (FeCl3) | 0.5 | 47 |
| Hg2+ (HgCl2) | 1.0 | 0 |
| Ni2+ (NiCl2) | 1.0 | 51 |
| Cu2+ (CuCl2) | 1.0 | 0 |
| Cd2+ (CdCl2) | 1.0 | 0 |
| EDTA | 0.5 | 117 |
| L-Cysteine | 25 | 148 |
| L-Histidine | 25 | 114 |
| Glutathione (reduced) | 0.5 | 136 |
| Thiourea | 0.5 | 114 |
| Thioacetic acid | 0.5 | 106 |
| Thioacetamide | 0.5 | 110 |
| 2-mercaptoethanol | 0.5 | 112 |
| Dithiothreitol | 0.5 | 86 |
| p-chloro mercury benzoic acid | 0.5 | 0 |
| Iodoacetamide | 0.5 | 0 |
| Sodium dodecyl sulfate | 2.0 | 119 |
| 3.0 | 22 | |
| Urea | 2.0 | 108 |
| H2O2 | 1.0 | 24 |