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. 2009 Nov 1;2010:165878. doi: 10.4061/2010/165878

Table 3.

Kinetic constants of partially purified asparaginase.

Substrate Linear regression (Lineweaver-Burkplot) Nonlinear regression (Michaelis-Menten equation)
Km Vmax Vmax/Km Km Vmax Vmax/Km
(mM) (μmoles mg−1min−1) (mM) (μmoles mg−1min−1)
L-asparagine 0.096 1632.6 17,006 0.098 1666.7 17,007
L-glutamine 2.86 4.08 1.426 3.04 4.74 1.559