Table 3.
Kinetic constants of partially purified asparaginase.
| Substrate | Linear regression (Lineweaver-Burkplot) | Nonlinear regression (Michaelis-Menten equation) | ||||
|---|---|---|---|---|---|---|
| Km | Vmax | Vmax/Km | Km | Vmax | Vmax/Km | |
| (mM) | (μmoles mg−1min−1) | (mM) | (μmoles mg−1min−1) | |||
| L-asparagine | 0.096 | 1632.6 | 17,006 | 0.098 | 1666.7 | 17,007 |
| L-glutamine | 2.86 | 4.08 | 1.426 | 3.04 | 4.74 | 1.559 |