Table II.
IFN-γ-independent genes induced by IL-12 (50 genes)a
| Common Name | GenBank | Map | WT—IL-12- Treated |
GKO—Not Treated |
GKO—IL-12- Treated |
Function |
|---|---|---|---|---|---|---|
| RANTES, CCL5 | AF065947 | 11 | 38.79 | 0.34 | 4.44 | Chemoattractant for Mϕ/DC, T/NK/basophil/eosinophil |
| CD6 Ag | U37543 | 19 | 14.97 | 0.70 | 19.14 | Binds CD166, activated leukocyte-cell adhesion molecule |
| CD8 Ag β-chain | AV316162 | 6 | 13.15 | 0.52 | 10.25 | Cytotoxic T and DC markers |
| Ig κ-chain variable 28 (V28) | M18237 | 6 | 11.51 | 0.61 | 17.59 | Ab component |
| Preproinsulin-like growth factor IA, Igf1 | X04480 | 10 | 9.86 | 1.05 | 5.61 | Growth hormone mediator |
| CXCL13, BLC, BCA-1 | AF030636 | 9.37 | 0.30 | 12.44 | Chemoattractant for naive B and activated CD4+ T cells | |
| Similar to ζ-chain (TCR)-associated protein kinase ZAP-70 | AI386093 | 1 | 8.56 | 2.30 | 9.61 | T cell activation signaling |
| Putative castaneus IgK chain gene, C-region, IgM | M80423 | 8.48 | 0.08 | 17.41 | Natural Ab | |
| CD3 Ag, δ polypeptide | X02339 | 9 | 7.27 | 0.96 | 6.12 | T lymphocyte marker |
| T cell receptor α chain | M16118 | 14 | 6.82 | 1.00 | 9.47 | T lymphocyte marker |
| Adipsin | X04673 | 10 | 5.83 | 0.33 | 4.46 | Complement D activity, activates alternative pathway |
| IL-18R1 | U43673 | 5.81 | 0.66 | 9.16 | Receptor for an inflammatory cytokine | |
| CD3-γ | M18228 | 4.84 | 0.74 | 7.52 | T lymphocyte marker | |
| Adipocyte-specific Fsp27 | M61737 | 4.27 | 0.10 | 3.23 | Cell death-inducing factor-like | |
| ζ-chain (TCR)-associated protein kinase ZAP-70 | U04379 | 4.25 | 0.95 | 4.80 | T cell activation signaling | |
| α-1 acid glycoprotein (Agp-1B) | M27008 | 4 | 4.21 | 1.39 | 2.61 | Serum acute phase protein |
| Apolipoprotein D | X82648 | 16 | 4.08 | 1.48 | 4.77 | Androgen-regulated, plasma lipid transport |
| Apolipoprotein C1 (Apoc1) | Z22661 | 7 | 3.69 | 0.75 | 2.01 | Mediator of lipid binding in plasma |
| Similar to human lipoprotein lipase | AA726364 | 8 | 3.69 | 0.71 | 2.38 | Hydrolyzes triglycerides in lipid metabolism |
| Stromal cell-derived factor, SDF-1, Sdf1a | L12029 | 3.41 | 0.33 | 2.83 | Chemoattractant for T/B/DC and CD34+ progenitor cells | |
| spi2/eb4 protease inhibitor | M64086 | 3.37 | 1.15 | 2.89 | Inhibitor of serine protease | |
| Activator of S phase kinase, DBF-4-related protein | AJ003132 | 3.33 | 0.35 | 2.26 | G1/S phase transition in cell cycle | |
| Monocyte chemoattractant protein 2 (MCP2), CCL8 | AB023418 | 11 | 3.30 | 0.16 | 1.46 | Chemoattractant for T/monocyte/basophil/eosinophil |
| ICAM-1 | M90551 | 9 | 3.00 | 0.44 | 1.94 | Cell adhesion/migration |
| c-myb | M12848 | 10 | 3.00 | 0.70 | 1.52 | Proto-oncogene |
| Lipoprotein lipase | M63335 | 8 | 2.80 | 0.53 | 2.36 | Hydrolyzes triglycerides in lipid metabolism |
| Stearoyl-CoA desaturase, ab, SCD, Scd-1 | M21285 | 19 | 2.79 | 0.52 | 2.65 | Synthesis of monounsaturated fatty acids |
| PGE receptor subtype EP2 | AB007696 | 14 | 2.73 | 0.23 | 2.16 | PGE2 signaling |
| Voltage-dependent Na+ channel β 1 subunit | L48687 | 2.65 | 1.31 | 2.67 | Regulates Na+ channels | |
| C57BL/6J ob/ob haptoglobin, Hp | M96827 | 8 | 2.68 | 0.42 | 1.91 | Serum acute phase protein |
| TCR germline β-chain gene constant region (CT) | M26056 | 2.59 | 1.00 | 3.61 | T cell marker and signaling | |
| Killer cell lectin-like receptor, subfamily D, member 1, CD94 | AF030311 | 2.59 | 0.59 | 3.24 | Cytotoxic killer cell inhibitory receptor | |
| Lck, protein tyrosine kinase | M12056 | 2.58 | 0.89 | 3.87 | Lymphocyte-specific signaling | |
| Pentraxin related gene, PTX3 | X83601 | 3 | 2.54 | 1.24 | 2.23 | Secreted pattern-recognition receptor |
| Nuclear matrix attachment DNA-binding protein SATB1 | U05252 | 17 | 2.49 | 0.66 | 4.06 | Regulator of chromatin remodeling |
| Killer cell lectin-like receptor subfamily C, member 1, NKG2A | AF095447 | 2.42 | 1.11 | 2.45 | Inhibitory receptor of cytotoxic cells | |
| Granzyme B | M12302 | 2.41 | 0.58 | 2.52 | Cytotoxic killing | |
| b allele; Ig active J chain | M90766 | 5 | 2.38 | 1.00 | 4.16 | Ab component |
| Nuclear orphan receptor LXR-α | AF085745 | 2.34 | 0.70 | 2.42 | cAMP-responsive transcriptional regulator | |
| Ig-like receptor PIRA3 (7M1) | U96684 | 2.34 | 0.93 | 1.91 | Mast cell activating | |
| Germ line gene fragment for µ-Ig C terminus (secreted form), Igh-6 | V00817 | 12 | 2.31 | 0.48 | 4.00 | Ab component |
| Plasma phospholipid transfer protein | U28960 | 2 | 2.31 | 0.73 | 1.58 | Suppressor of cancer cell |
| Lymphotoxin B | U16985 | 17 | 2.27 | 0.61 | 3.44 | Cytotoxicity |
| Serum amyloid A (SAA) 3 protein | X03505 | 7 | 2.25 | 0.80 | 1.95 | Serum acute phase protein |
| Stroma cell-derived factor, SDF-1 β | L12030 | 2.21 | 1.10 | 2.84 | Chemoattractant for T/B/DC and CD34+ progenitor cells | |
| Gpcr25, putative G protein-coupled receptor TDAG8 | U39827 | 12 | 2.11 | 0.66 | 1.40 | T cell death-associated gene |
| GM-CSF 2 receptor, β1, low affinity | M34397 | 15 | 2.10 | 0.60 | 1.96 | Growth and differentiation of granulocytes and Mϕ |
| Close homolog of L1, CHS-1-like protein | X94310 | 2.09 | 0.37 | 3.28 | Novel neural recognition molecule | |
| Adipocyte complement-related protein of 30 kDa,AdipoQ | U49915 | 2.06 | 0.34 | 2.07 | Regulates insulin sensitivity | |
| XCL1, lymphotactin | U15607 | 2.01 | 1.34 | 2.71 | Chemoattractant for NK and T lymphocytes |
a
Genes are selected based on the following criteria: 1) they must be expressed more than two times than the respective control samples (untreated wild type or GKO) to qualify as IL-12-induced genes; 2) their expression detection must be statistically valid as present with detection p< 0.05; 3) IFN-γ-dependent genes are defined as those whose expression must be induced by IL-12 in GKO mice less than two times compared with untreated GKO mice; and 4) IFN-γ-independent genes are defined as those whose expression must be induced by IL-12 in GKO mice greater than two times compared with untreated GKO mice. GenBank accession numbers are given. Chromosomal locations (map) are given when they are known. The numbers in columns 4–6 are relative fold of expression over the baseline (control untreated, wild-type group), which is arbitrarily set as 1.