FIGURE 3.

Utp25 is required for growth and for 18S pre-rRNA processing. (A) Genetic depletion of Utp25 after 24 h of growth in nonpermissive glucose medium impairs cell growth. (Inset) Utp25 and Utp6 (Dragon et al. 2002) levels (*) are reduced after genetic depletion for 24 h. Total yeast extract, separated on the same SDS-PAGE gel, was analyzed by Western blotting with an anti-HA antibody. A 50-kDa nonspecific cross-reacting band is also seen (•). (B) Genetic depletion of Utp25 reveals an 18S rRNA maturation defect. The parental strain YPH499 along with GAL∷3HA-UTP6 (Dragon et al. 2002) are shown as controls. Oligonucleotide hybridization probes c, b + e, and a + y are depicted in Figure 1 as lowercase letters above the rRNA schematic.