FIGURE 7.

The helicase-like domain of Utp25 mediates protein–protein interactions with Utp3. The full-length and N- and C-terminal truncations of Utp25 were cloned as bait, and the full-length Utp3 was cloned as prey, transformed into yeast and screened by yeast two-hybrid in an all-against-all mating array. Yeast cells were assayed for growth on selective medium, as described in the legend to Figure 6. The locations of the C-terminal truncations of Utp25, along with the N-terminal truncation corresponding to the helicase-like DUF1253 motif, are shown. The C-terminal truncations lie outside of the predicted location of the degenerate helicase motifs.