FIGURE 2.

Identification of transcription start sites by 5′ RACE-PCR. (A) Localization and frequencies of various TSSs identified by 5′ RACE-PCR in 54-O cells, a cell line isolated from a lymphoma induced by GaHV-2. (B) Localization and frequencies of various TSSs identified by 5′ RACE-PCR in LMH cells transfected with the P1Δ3′ pGL3 vector (luciferase gene under the control of the P1Δ3′ promoter). Transcription start sites are shown in bold for RNA from 54-O cells and are underlined for LMH cells transfected with the P1Δ3′ pGL3 vector. Each TSS is labeled with the number of initiations found. The DNA sequence represents GaHV-2 genome, with the last 60-bp repeat highlighted in gray.