FIGURE 6.

Direct association of p53 with the LAT-miRNA promoter The PCR amplification is carried out using primers pair M645 and M735 (Table 1). Electrophoretic analyses of the PCR amplification products, using immunoprecipitated DNA (IP) or total input DNA (Input), are shown. Mouse IgG1 antibodies (left column) were used in ChIP assays as a negative control. The right column shows the PCR amplification products from DNA immunoprecipitated with p53 antibodies HP64. LAT-miRNA-promoter specific primers amplified products of various sizes from 154 bp to more with an increment of 60 bp corresponding to one 60-bp repeat.