FIGURE 7.
Tumor protein p53 transactivates the [60 bp]2 minimal promoter SAOS-2 cells were cotransfected with the minimal promoter [60 bp]2 and an empty pcDNA3.1(+) expression plasmid and an expression plasmid for chicken p53 (pcCHP53) (A) or plasmids encoding wild-type human p53 (pHP53SN) and mutated human p53 (pHP53CX3) (B). Luciferase activities following cotransfection with pcCHP53 and pHP53SN were standardized with respect to those of pcDNA3.1 (A) and pHP53CX3 (B), respectively. PRL-TK (A) and pRL-CMV (B) vectors were used to normalize the transfection rate. Each value corresponds to the mean of at least three independent assays performed simultaneously. Error bars indicate the SEM for three replicates.