FIG 2 .
MHV upregulates MDA5 and RIG-I expression to higher levels in BMM than in BMDC. (A) Basal mRNA levels of MDA5 and RIG-I in mock-infected BMM and BMDC were measured by real-time qPCR. Values that are significantly different (P < 0.05) from the value for BMM are indicated by an asterisk. (B and C) mRNA levels of MDA5 and RIG-I in BMM (B) and in BMDC (C) at different times p.i. were measured by real-time qPCR. CT ratios to HPRT were calculated as described in Materials and Methods. MDA5 and RIG-I were upregulated to a greater extent in BMM than in BMDC (note the differences in scale). Two or three replicates were performed in each experiment, and one of three independent experiments is shown.