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Introduction of the TEV cleavage site to the C terminus increased pore-forming activity. OprF (wt, F160I, or CS) was modified by the addition, at the C terminus, of a factor X/TEV cleavage site (IEGRNLYFQS), followed by a hexahistidine tag. These derivatives of pKY plasmids were expressed in HN705 without IPTG induction, and the Opr proteins were purified and assayed by a liposome-swelling assay with l-arabinose. In some plasmids, the cleavage site sequence was altered.
