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. Author manuscript; available in PMC: 2010 Oct 19.
Published in final edited form as: Int Immunol. 2006 Jun 1;18(8):1243–1251. doi: 10.1093/intimm/dxl055

Fig. 2.

Fig. 2

Expanded Vδ2 T cells uniformly express cytoplasmic RANTES. In vitro-expanded Vδ2 T cells were stained with PE-labeled antibodies to Vδ2 TCR chain, permeabilized, and then stained with anti-RANTES FITC-labeled antibodies. IgG isotype controls are presented a presented in panel A. More than 85% of cells were positive for Vδ2 (B) and double positive for Vδ2 and RANTES (C). A small population of RANTES single-positive cells were also detected (C). We confirmed the presence of cytoplasmic RANTES (green) in Vδ2-positive cells (red) by confocal microscopy.