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Secreted RANTES is a full-length protein. An anti-RANTES antibody was used to capture a synthetic, full-length RANTES (A) or chemokine released from Vγ2Vδ2 T cells after PHA stimulation (B). The protein masses were nearly identical, and were clearly distinct from the mass (7663.4) expected for truncated RANTES (indicated in B). A control antibody (C) did not capture protein in this mass range from the Vγ2Vδ2 T cell supernatant.
