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. 2010 Jul 14;83(4):663–675. doi: 10.1095/biolreprod.110.084889

FIG. 5.

FIG. 5.

RT-PCR validation of expression of cell-cycle genes during spermatogenesis. A) Validation of spermatogenesis-specific expression of meiosis-specific cell-cycle genes. Expression of five cell-cycle genes annotated as meiosis-specific (Slxl1, Pou5f2, Gm7958, 1700025K23Rik, and 1700009N14Rik) was examined in spermatogonia (G), spermatocytes (C), and spermatids (T), along with liver (L), intestine (I), muscle (M), and spleen (S) as somatic controls to confirm tissue-specific expression of these genes. B) Validation of spermatogenic cell expression of ubiquitous cell-cycle genes. Expression of nine cell-cycle genes annotated as ubiquitously expressed (Bccip, Mtap9, Dusp1, Rpa2, Ppp6c, Gas2, Ccny, Cntrob, and Ppp1ca) was examined in spermatogonia (G), spermatocytes (C), and spermatids (T), along with liver (L) to confirm ubiquitous expression in spermatogenic cells and in a somatic tissue. Gapdh expression was used to normalize efficiency of cDNA synthesis and as a positive control for ubiquitous expression. NT, no template control.