FIG. 3.

Representative dark-field photomicrographs showing NPVF mRNA-expressing cells (GnIH; as reflected by the presence of white clusters of silver grains) in the PVN/DMN region from rhesus macaques at the luteal phase (A), early follicular phase (B), and late follicular phase (C). Quantification of NPVF mRNA shows the number of NPVF mRNA-positive cells per section was unchanged over the cycle, but the number of grains per NPVF cell was significantly reduced in luteal-phase animals. Values are presented as the mean ± SEM (n = 3–4 per group). 3V, third ventricle. Bars = 200 μm. *P < 0.05.