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. 2010 Oct;38(10):1892–1899. doi: 10.1124/dmd.110.033548

Fig. 2.

Fig. 2.

Substrate concentration dependence for the O-methylation of 2-OH-E2 and 4-OH-E2 catalyzed by four human placental cytosolic COMT preparations. The incubation mixture consisted of 0 to 36 μM 2-OH-E2 or 4-OH-E2, 250 μM AdoMet (containing 0.2 μCi of [methyl-3H]AdoMet), 0.5 mg/ml placental cytosolic protein, 1 mM dithiothreitol, and 1.2 mM MgCl2 in a final volume of 0.5 ml of Tris-HCl buffer (10 mM, pH 7.4). The incubations were performed at 37°C for 10 min. Each point is the mean of duplicate determinations (average variation <5%).