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YKU70/YKU80 over-expression sensitizes sae2Δ and mre11-nd mutants to IR. (A) Spot assays and (B) survival plots of wild type, sae2Δ and mre11-nd mutants transformed with empty or YKU70/YKU80 over-expressing vectors. Exponentially growing cells in SC-Ura to maintain selection of the plasmids were 1:10 serially diluted, spotted onto SC-Ura plates and exposed to IR. The means from at least three experiments are presented, error bars indicate s.d.; *P=0.01, **P=0.009.
