Figure 6.

Telomere-associated phenotypes of sae2Δ sgs1Δ yku70Δ mutants. (A) Schematic representation of the telomeric Y′ elements and TG repeats. XhoI liberates a wide band of ∼1.3 kb in wild-type cells, which is used to evaluate telomere repeat length in other genetic backgrounds. (B) Southern blot analysis of XhoI-digested genomic DNA as detected with a Y′ probe. (C) Telomere length analysis after induction of YKU70 expression with galactose in the yku70Δ and sae2Δ sgs1Δ yku70Δ mutant. (D) Viability and genotypes of spores derived from diploids heterozygous for the indicated mutations.