Figure 4.

Armi localizes to Yb bodies and Yb is an essential pathway component. (A) Shown are immuno-fluorescence stainings for Armi (red) and Piwi (green) in somatic follicle cells. DNA was labelled with DAPI (blue). (B) Immuno-fluorescence staining for Armi (red), Tubulin (green) and DNA (blue) in OSCs. (C) Immuno-fluorescence analysis of Armi (red) in the follicular epithelium of an egg chamber containing a Notch dsRNA-expressing clone (clone marked with GFP (green) and clone borders indicated by the dashed line). DAPI staining (blue) confirms that Notch knockdown impairs endo-replication. (D) Co-labelling of Armi (red) and DCP1-GFP (green) in somatic follicle cells. (E) Co-labelling of Armi (red) and Yb (green) in somatic follicle cells. (F) Co-immuno-precipitation of endogenous Armi with FLAG-tagged Yb. Shown are western blots against FLAG to detect Yb (top) and against endogenous Armi (bottom). Control cells did not express FLAG-Yb. (G) Shown are β-Gal stainings of ovarioles expressing the gypsy reporter and two different dsRNA hairpins against fs(1)Yb with tj-GAL4. (H) Shown are fold changes in steady-state RNA levels (n=3) of ZAM, Tabor, HeT-A and TART retro-elements in ovaries in which fs(1)yb and aub (control) were knocked down through tj-GAL4-mediated RNAi. Values are normalized to a tj-GAL4/+ control.