FIG. 3.

The rAAV2 RSM was run on SDS–polyacrylamide gels under both reducing and native conditions and then stained with (a) SYPRO ruby or (b) silver stain. An in-house rAAV2 standard was run as a positive control and buffer as a negative control. The lanes for each gel are as follows: (1) benchmark ladder (unstained or prestained)—reduced; (2) negative control—reduced; (3) AAV reference material—reduced; (5) positive control—reduced; (6) benchmark ladder (unstained or prestained)—native; (7) negative control—native; (8) AAV reference material—native; (10) positive control—native.