Table 3.

rAAV2 Reference Standard Material Raw Characterization Data

Laboratory	Replicate	Particle titer (ELISA) (pt/ml)	Genome titer (qPCR) (VG/ml)	Transducing titer (green cells) (GFU/ml)	Infectious titer (TCID50) (IU/ml)a
A	1	1.08 × 1012	4.68 × 1010	1.27 × 109
	2	1.26 × 1012	8.77 × 1010	1.26 × 109
B	1	8.25 × 1011	7.03 × 1010	3.63 × 108	6.32 × 109
	2	8.28 × 1011	7.58 × 1010	1.00 × 108	1.36 × 109
	3		9.39 × 1010	7.15 × 107	2.00 × 109
C	1	7.70 × 1011	8.60 × 1010	9.80 × 106
	2	6.05 × 1011	4.19 × 1010
D	1	8.80 × 1011	1.01 × 1010	1.70 × 109	1.02 × 1010
	2	7.83 × 1011	3.71 × 1010	2.77 × 109	6.96 × 109
E	1	1.16 × 1012	1.58 × 1010
	2	1.04 × 1012	1.14 × 1010
	3		1.61 × 1010
F	1	1.66 × 1012	2.17 × 1010	6.60 × 108
	2	1.01 × 1012	2.12 × 1010	5.70 × 108
G	1	1.90 × 1012	1.13 × 1010	4.02 × 108	3.23 × 109
	2	9.39 × 1011	1.30 × 1010		2.67 × 109
H	1		2.04 × 1010
	2		2.13 × 1010
I	1	8.56 × 1011	5.93 × 1010	2.20 × 107
	2	7.08 × 1011	6.10 × 1010	2.95 × 107
J	1	1.24 × 1012	3.39 × 1010	1.89 × 109	1.65 × 1010
	2	1.07 × 1012	4.49 × 1010	1.22 × 109	2.42 × 1010
K	1	5.29 × 1011	3.72 × 1010	2.01 × 108
	2	7.75 × 1011	3.62 × 1010	2.04 × 108
L	1	8.13 × 1011	1.31 × 109	5.96 × 108	1.50 × 109
	2	4.99 × 1011	1.63 × 1010	6.48 × 108	6.96 × 108
M	1	1.11 × 1011	1.16 × 1010	8.88 × 108	2.00 × 109
	2	1.25 × 1011	1.53 × 1010	4.52 × 108	1.50 × 109
N	1	3.93 × 1011	1.47 × 1010	3.82 × 108	2.00 × 1010
	2	5.59 × 1011	7.63 × 109	2.62 × 108	7.66 × 109
	3	9.58 × 1011	4.24 × 109		2.40 × 109
O	1	1.06 × 1012	6.04 × 1010
	2	1.09 × 1012	1.27 × 1011
P	1	8.02 × 1011	4.20 × 1010		7.66 × 109
	2	7.74 × 1011	5.10 × 1010		6.96 × 109
	3		4.82 × 1010		9.28 × 109
Mean		9.43 × 1011	3.82 × 1010	6.94 × 108	7.00 × 109
Standard deviation		3.19 × 1011	2.97 × 1010	7.03 × 108	6.70 × 109

^aFour replicate test results were omitted because of documented experimental deviation from the posted protocols.