Fig. 2.

mRNA expression of podocyte markers in podocytes transformed by CDK4 is distinct from podocytes transformed by thermosensitive SV40 large T antigen. Expression of nephrin (A), synaptopodin (B), Wilms tumor 1 (WT1; C), P-cadherin (D), podocalyxin (E), or nonmuscle myosin heavy chain IIA (MYH9; F) mRNA in primary podocytes (primary-p), podocytes transformed by CDK4 (CDK4-p), or podocytes transformed by tsA58U19 (tsT-p) under growth-permissive condition (GP), growth-restricted condition (GR) day 5, or GR day 14 grown in confluence was analyzed by quantitative RT-PCR. A: nephrin mRNA was diminished in tsT-p in contrast to CDK4-p in which it was preserved. B: synaptopodin mRNA was suppressed in CDK4-p and tsT-punder GP and GR at day 5, whereas it was retained in tsT-p under GR at day 14. D: podocalyxin mRNA was higher in CDK4-p and tsT-p under GR day 14 compared with primary podocytes. C, D, F: expression of WT1, P-cadherin, and MYH9 mRNA was mostly preserved in both CDK4-p and tsT-p. The data were normalized to β-actin mRNA. Data are presented as means ± SD of triplicate cultures, relative to primary podocytes. ***P < 0.001.