Fig. 6.

Detection of ROMK surface membrane protein in biotinylated rat kidneys. A: each lane was loaded with neutravidin eluates obtained from 500 μg total membrane protein. In the 2 lanes on the right, the kidneys were perfused with biotinylating reagent. In the 2 lanes on the left, the kidneys were perfused without the biotinylating reagent. B: blot similar to that in A of neutravidin eluates from kidneys perfused with biotinylating reagent. The right lanes were loaded with samples treated with PNGase F as described in methods. The left lanes were loaded with control samples treated identically but without PNGaseF.