Fig. 3.

MyD88 deficiency enhances the KC-induced downregulation of neutrophil CXCR2 expression. Bone marrow-derived neutrophils from both WT and MyD88^−/− mice were incubated with or without the indicated KC concentrations at 37°C for 30 min. Cells were stained and analyzed in flow cytometry and gated on Gr-1 and CXCR2. A: accumulated data of neutrophil CXCR2 expression presented as the percentage of the control cells without KC treatment. Each error bar represents mean ± SD of 4 separate neutrophil preparations. B: a representative plot of flow cytometry illustrating neutrophil CXCR2 expression in the control cells and the cells treated with 50 nM KC from WT and MyD88^−/− mice.