Fig. 6.

MyD88^−/− mice have marked attenuation in myocardial neutrophil recruitment after I/R. Twenty-four hours after 60 min of left anterior descending coronary artery (LAD) ligation, the hearts were isolated, perfused, and digested. After removal of the large cardiomyocytes through filtration, 50% of total cells were loaded onto flow cytometry and gated on Gr-1 and CXCR2. A: total Gr-1⁺ cells as measured by flow cytometry. Each error bar represents mean ± SD of 4 mice. A small number of neutrophils were recovered from the sham-operated hearts as indicated by the line. B: a representative example of flow cytometry plots of myocardial infiltrating cells from sham, WT-I/R, and KO-I/R mice. FSC, forward scatter; SSC, side scatter.