Fig. 7.

Toll-interleukin-1 receptor (TIR)-domain-containing adaptor protein-inducing interferon-β-mediated transcription factor (Trif) deficiency has no impact on myocardial neutrophil recruitment and KC-induced CXCR2 downregulation. Twenty-four hours after 60 min of LAD ligation, the isolated hearts were perfused and digested. After removal of the large cardiomyocytes, 50% of total cells were loaded onto flow cytometry and gated on Gr-1 and CXCR2. A: total Gr-1⁺ cells as measured by flow cytometry from the hearts subjected to I/R. Each error bar represents mean ± SD of 3 mice. B: a representative example of flow cytometry plots of myocardial infiltrating cells from WT-I/R and Trif-KO-I/R mice. C: accumulated data of neutrophil CXCR2 expression presented as the percentage of the control cells without KC treatment. Each error bar represents mean ± SD of 3 separate neutrophil preparations. D: a representative plot of flow cytometry illustrating neutrophil CXCR2 expression in the control cells and the cells treated with 50 nM KC from WT and Trif-KO mice.