Fig. 1.

Expression of Src homology-1 phosphatase (SHP-2) constructs in REN cells and REN cells expressing human platelet endothelial cell adhesion molecule-1 (PECAM-1) (REN-HP). A: three constructs that were expressed in REN and REN-HP cells: wild-type SHP-2 (WT), phosphatase-inactive SHP-2 (CS), and SHP-2 with tyrosines 542 and 580 mutated to phenylalanines (2YF). B: cell lysates from REN and REN-HP cells expressing the various SHP-2 constructs, demonstrating both the presence of the endogenous SHP-2 (lower molecular weight band) and the transduced SHP-2 in the transfectants expressing a SHP-2 construct. (The data are from the same experiment and the same gel for each antibody and are representative of 3 experiments). C: intracellular SHP-2 phosphatase activities of each cell line as determined by the amount of phosphate released by 5 μg of cell lysate (see materials and methods) (n = 2). (The phosphatase data are representative of 2 experiments).