Fig. 8.

Cord/tube formation on Matrigel by REN cells and REN-HP cells expressing SHP-2 constructs. Shown are REN cells (A–D) and REN-HP cells (E–H), expressing no SHP-2 constructs (A and E), WT-SHP-2 (B and F), CS-SHP-2 (C and G), or 2YF-SHP-2 (D and H) plated on Matrigel. REN cells did not form cord/tube-like networks on Matrigel, whereas all of the REN-HP cell transfectants did form cord/tube-like networks at comparable levels. Cord/tube formation was quantitated using image analysis (I). There were no significant differences between the various cell lines (n = 6). The data presented are representative of 3 experiments.