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HepG2 cells were treated with 20 µM 4-HNE for the indicated time points at 37°C. Total protein lysates were collected as described under Materials and methods section. The lysates were analyzed by western blotting for p53, p-p53 (Ser15), p21, JNK, Bax and Bcl-xL. β-actin was used as a loading control.
