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. Author manuscript; available in PMC: 2010 Oct 19.
Published in final edited form as: Methods Enzymol. 2010 Mar 1;471:291–317. doi: 10.1016/S0076-6879(10)71016-8

Figure 4.

Figure 4

Phosphorylation of Ypd1. (a) Schematic depicting strategy for obtaining phosphoryated Ypd1 for subsequent in vitro biochemical studies. Bead-bound GST-Sln1-HK-Rec is co-incubated with [γ-32P]ATP and Ypd1 and the products separated by centrifugation. (b) An example phosphotransfer experiment in which isolated 32P-labeled Ypd1 (WT) and a number of Ypd1 mutants were examined for their ability to transfer phosphoryl groups to the Ssk1-Rec domain (reproduced from Janiak-Spens et al., 2000).