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Combined knockdown of RP2 and PKD2 increases incorrect heart looping. (A) RP2 and PKD2 morpholinos were injected into CMLC:GFP zebrafish embryos at the indicated dosages. Heart looping was analyzed by fluorescence microscopy 48 hpf. (B) RP2 morphants have normal Kupffer's vesicle development. Zebrafish embryos were injected with either mismatch or RP2 morpholinos. Embryos were fixed at the 10–12 somite stage and stained for acetylated tubulin (red).
