Fig. 6.

UDCA and deoxycholic acid (DCA) modulate miR-21 expression in proliferating HepG2 cells and primary rat hepatocytes, respectively. Cells were incubated with 100 μM UDCA, or DCA, or no addition (control) for 4, 16, 24, 40, and 48 h in primary rat hepatocytes (A), or 2, 4, 6, 24, and 48 h in HepG2 cells (B). Total RNA was obtained for real-time RT-PCR analysis as described in materials and methods. Results are expressed as means ± SE for at least 3 different experiments. *P < 0.01 from control 4 h and †P < 0.05 from the respective control.