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. 2010 Jul 22;299(4):G980–G989. doi: 10.1152/ajpgi.00080.2010

Fig. 6.

Fig. 6.

Effects of IL-6 on apoB mRNA and apoB mRNA decay by Northern blotting. A: RH were incubated with increasing concentrations of IL-6 (0–1 nM) for 16 h, and RNA was isolated and analyzed by Northern blotting. B: apoB mRNA abundance relative to cyclophilin mRNA was quantified by phosphoimager analysis. This is a representative experiment that was repeated with essentially identical results. C: average percentage change ± SD in apoB mRNA abundance with IL-6 treatment normalized to cyclophilin in 6 independent rat liver experiments and normalized to 18S rRNA in 4 independent rat liver experiments. *The mean percent of IL-6 mRNA relative to normalization control is significantly greater than control after normalization. D: to assess cellular stability of apoB mRNA, RH were incubated with and without IL-6 (0.5 nM) followed by addition of 5,6-dichlorobenzimidazole (DRB) to arrest mRNA synthesis. Cellular RNA (5 plates/time point) was isolated before addition of DRB (0 h) and various times thereafter (1, 6, 12, 18, and 24 h). Cellular apoB mRNA levels were determined by Northern blotting and normalized using 18S rRNA hybridization signal in 3 independent rat liver experiments. E: average percent of cellular apoB mRNA remaining ± SD (n = 3) at each time point with IL-6 treatment (○) and without IL-6 treatment (●) was plotted against time after addition of DRB.