Fig. 3.

TNF-α inhibits SMCT1 function and expression in IEC-6 cells. The cells grown on transwell inserts were treated basolaterally with indicated concentrations of TNF-α for 24 h. A: Na⁺-dependent ¹⁴C-butyrate uptake was measured in control or TNF-α-treated cells. B: total RNA was isolated from control or TNF-α-treated cells, and SMCT1 mRNA was measured by real-time quantitative PCR. Values are means ± SE; n = 3. *Different from control, P < 0.001.