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Oral administration of live LP increases SMCT1 mRNA and protein in mouse ileum and colon. C57BL/6 mice were gavaged with live LP in sterile PBS containing 1 × 10−9 colony-forming units/ml of the bacteria. After 24 h, mice were killed, intestine removed, and mucosa was scraped from ileum and colon for RNA isolation and preparation of cell lysate. SMCT1 mRNA was measured by real-time PCR using gene specific primers (A) and SMCT1 protein levels by immunoblotting (B).
