Fig. 1.
Effect of methylprednisolone on the intestinal NHE8 protein expression in rats. Brush-border membrane vesicles (BBMVs) were isolated from the intestinal mucosa of control or methylprednisolone-treated rats. BBMV protein (30 μg) was loaded on SDS-PAGE and immunoblotted. Rat NHE8 antibody and β-actin antibody were used to detect NHE8 and β-actin, respectively. The expression of NHE8 protein was calculated by dividing the optical density of the NHE8 band over that of the β-actin band. Bars show the NHE8 protein expression indicated as means ± SE in the sum of 4 independent experiments. *P ≤ 0.01 for control (CT) groups vs. methylprednisolone (MP) groups. Inset: the corresponding Western blot image. A: effect of methylprednisolone on NHE8 protein expression in rat jejunum. B: effect of methylprednisolone on NHE8 protein expression in rat ileum.