Fig. 2.
Effect of methylprednisolone on the intestinal NHE8 mRNA expression in rats. RNA was isolated from the intestinal mucosa of control (CT) or methylprednisolone-treated (MP) rats and used for Real-Time PCR. NHE8 mRNA and TATA binding protein (TBP) mRNA were amplified with rat-specific NHE8 and TBP primers. The changes in NHE8 gene expression were analyzed by the comparative cycle threshold (Ct) method. Data are means ± SE from a total 18 rats (9 for the methylprednisolone group, 9 for the control group). *P ≤ 0.01 for the control group vs. the methylprednisolone group. Left: effect of methylprednisolone on NHE8 mRNA expression in rat jejunum. Right: effect of methylprednisolone on NHE8 mRNA expression in rat ileum.