Fig. 4.

Effect of dexamethasone on human NHE8 gene promoter activity. Cells were cotransfected with pRL-CMV and pGL3 basic (pGL3b) or human NHE8 promoter constructs (pGL3b/−671, pGL3b/−89, or pGL3b/−32). Dexamethasone was applied 18 h before measuring promoter activities. Promoter activity is shown as a relative activity, a ratio of firefly luciferase activity driven by NHE8 promoter over Renilla luciferase activity driven by CMV promoter. The degree of inhibition is shown as the ratio of luciferase activity in dexamethasone-treated cells (shaded bars) over luciferase activity in vehicle-treated cells (solid bars). Results are means ± SE from 6 separate experiments. *P < 0.01 for control vs. dexamethasone treatment.