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Narrowing down the DNA region involved in dexamethasone regulation. Nuclear proteins were isolated from dexamethasone-treated (D) and nontreated (C) Caco-2 cells. Probes 1, 2, 3, and 4 for gel mobility shift assays (GMSAs) were made with [32P]ATP end-labeled DNA oligos 1, 2, 3, and 4, respectively. The oligos span the proximal promoter region of the human NHE8 gene. Results shown are representative of 3 separate experiments.
