Fig. 5.

PTPN2 knockdown does not affect EGF-induced phosphorylation of ERK1/2. T₈₄ cells transfected with either control siRNA or PTPN2 siRNA were treated with EGF (100 ng/ml) for 5 min. Analyses were performed using whole cell lysates. Representative Western blots show phosphorylation and expression of ERK1/2. The densitometric analysis of 3 similar experiments is shown by the histogram below. Data are shown as a percentage of control. Significant difference vs. the respective control: **P < 0.01.