Fig. 1.
Effects of 20-hydroxyeicosatrienoic acid (20-HETE) on L-type Ca2+ current (ICa,L) in rat isolated cardiomyocytes. A: concentration-dependent response of peak ICa,L to 20-HETE (from 10 to 100 nM) in rat isolated cardiomyocytes. Wash, washout. Results are means ± SE; n = 13. *P < 0.05 compared with vehicle (Veh) control (Con). B: representative current-voltage curve recorded before and after the administration of 20-HETE (100 nM). C: representative examples of ICa,L recorded from isolated cardiomyocytes elicited by 300-ms depolarizing pulses from a holding potential of −50 mV to test potentials of +50 mV before and after the administration of 20-HETE (10 nM). D: representative current-voltage curve recorded before and after the administration of nifedipine (Nife; 2 μM). E: bar graph summarizing the effects of Nife (2 μM) on 20-HETE-induced activation of ICa,L in isolated cardiomyocytes. Results are means ± SE; n = 10. *P < 0.001 compared with Con. F: time-dependent response of peak ICa,L to 20-HETE in rat isolated cardiomyocytes. ICa,L was recorded in adult rat cardiomyocytes treated with 20-HETE (100 nM) at the time points indicated in the graph. Results are means ± SE; n = 9. *P < 0.001 compared with Con.