Fig. 1.

Coimmunoprecipitation of endothelial nitric oxide synthase (eNOS) with heat shock protein (Hsp)90 for pulmonary resistance arteries (PRAs) from piglets raised in normoxia or hypoxia for 3 days (A and B) or 10 days (C and D). PRA protein homogenates (n = 5 different piglets in each group) were immunoprecipitated (IP) with either anti-Hsp90 (A and C) or anti-eNOS (B and D). The resultant immunoprecipitates were subjected to immunoblot (IB) analysis using antibodies against eNOS and Hsp90. *Significantly different from the comparable-age normoxic group (P < 0.05 by unpaired t-test).