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. 2010 Aug 6;299(4):H1190–H1204. doi: 10.1152/ajpheart.01207.2009

Fig. 2.

Fig. 2.

Effect of ACh stimulation on coimmunoprecipitation of eNOS with Hsp90 under untreated (vehicle) or geldanamycin (GA)-treated conditions for PRAs from piglets raised in hypoxia for either 3 days (A and B) or 10 days (C and D). PRA protein homogenates were immunoprecipitated with either anti-Hsp90 (A, 3-day hypoxic piglets, n = 7 vehicle-treated or untreated piglets and 7 GA-treated piglets; and C, 10-day hypoxic piglets, n = 7 vehicle-treated or untreated piglets and 7 GA-treated piglets) or anti-eNOS (B, 3-day hypoxic piglets, n = 5 vehicle-treated or untreated piglets and 5 GA-treated piglets; and D, 10-day hypoxic piglets, n = 7 vehicle-treated or untreated piglets and 7 GA-treated piglets). The resultant immunoprecipitates were subjected to immunoblot analysis using antibodies against eNOS and Hsp90. The summary densitometric data shows that ACh stimulation had no effect on the coimmunoprecipitation of eNOS with Hsp90 for untreated and GA-treated PRAs from either group of hypoxic piglets.