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Coimmunoprecipitation of Hsp90 with prostacyclin synthase (PGIS) for PRA homogenates from a piglet raised in normoxia for 3 days. Different amounts of total protein were immunoprecipitated with anti-Hsp90 or an isotype-specific IgG (A) or with anti-PGIS or an isotype-specific IgG (B). The resultant immunoprecipitates were subjected to immunoblot analysis using antibodies against PGIS and Hsp90. +PGIS obtained from Cayman Chemicals, which was used as a positive control.
